Automated Cell Differentiation in Multispectral Microscopy

This paper presents a multispectral microscopy system for differential cytology. While conventional practices rely on the analysis of grey scale or RGB color images, presented system uses thirty one spectral bands for analysis. Algorithms designed to enable image segmentation, feature extraction, and classification are presented. Results are presented for the problem of discriminating among four cell types. In addition, classification performance is compared to the case where multispectral information is not taken into consideration. Results show that the developed system and the use of multispectral information along with morphometric information extracted from spectral images can significantly improve the classification performance and aid in the process of cell differentiation. Key–Words: Multispectral Microscopy, Differential Cytology, Cell Classification